Team:Kyoto/CiC/Notebook/1019-1023
From 2009.igem.org
- 0907-0911
- constructing HIV-TAT-(LALAAAA)3-generator
- 0914-0918
- constructing HIV-TAT-(LALAAAA)3-generator
- 0921-0925
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 0928-1002
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1005-1009
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1012-1016
- Observation
- 1019-1023
- Observation
1019-1025 :Obserbation
Monday, 19 October
To Do
- subgoal A --- Confirmation of function of TAT-liposome
- observation by microscope
- Subgoal B --- Liposomes with Mitochondrial Translocases
- Mitochondoria isolation
- expressing sig-GFP in vitro with mitochondoria
- Subgoal C --- sig-EGFP expression in HeLa
- transfomation
Results
subgoal A Confirmation of function of TAT-liposome
observation by microscope
Subgoal B --- Liposomes with Mitochondrial Translocases
Mitochondoria isolation
- expressing sig-GFP in vitro with mitochondoria
Subgoal C --- sig-EGFP expression in HeLa
sig-EGFP was made a localization in mitochondoria. See the results.
Tuesday, 20 October
To Do
Results
Wednesday, 21 October
To Do
Results