Team:Newcastle/IntroductoryLabwork/1 July 2009
From 2009.igem.org
(Difference between revisions)
(New page: {{:Team:Newcastle/CSS}} {{:Team:Newcastle/Header}} {{:Team:Newcastle/Left}} __NOTOC__ ===Lab session: 1st July=== [[Image:Team Newcastle iGem 2009 - Lab 01-07-09 no 6.JPG|thumb|right|Jess ...) |
(→Lab session: 1st July) |
||
Line 6: | Line 6: | ||
[[Image:Team Newcastle iGem 2009 - Lab 01-07-09 no 6.JPG|thumb|right|Jess streaking some agar plates]] | [[Image:Team Newcastle iGem 2009 - Lab 01-07-09 no 6.JPG|thumb|right|Jess streaking some agar plates]] | ||
[[Image:Team Newcastle iGem 2009 - Lab 01-07-09 no 9.JPG|thumb|right|Jane applying aseptic technique]] | [[Image:Team Newcastle iGem 2009 - Lab 01-07-09 no 9.JPG|thumb|right|Jane applying aseptic technique]] | ||
- | Things we need for today: | + | '''Today's introductory lab session saw the team progress with the next steps in the [https://2009.igem.org/Team:Newcastle/Project/Labwork/PhilsProtocols#Preparing_Competent_E.coli_cells_for_Heat_Shock making competent cells procedure] (i.e. steps 2 and 3 in the 'Pre-culture section). The team also looked at the future steps involved in the protocol and prepared solutions and equipment accordingly. This lab session (as well as the previous one) taught the team the importance of planning, organisation and time management. |
+ | |||
+ | ====Things needed for today's session==== | ||
+ | Things we need for today include: | ||
*LB | *LB | ||
*Containers | *Containers | ||
*Sterile tubes | *Sterile tubes | ||
- | *Space in incubator for tonight | + | *Space in orbital shaking incubator for tonight |
- | + | ||
+ | ====Progressing with making competent cells==== | ||
* We plated out single cultures from yesterday's plates for a practice. | * We plated out single cultures from yesterday's plates for a practice. | ||
* We inoculated 2ml of LB with single cultures from yesterday's plates and put them in the shaking incubator. | * We inoculated 2ml of LB with single cultures from yesterday's plates and put them in the shaking incubator. | ||
Line 18: | Line 21: | ||
- | + | ====Preparations for next lab session==== | |
+ | =====Considerations===== | ||
*Have we used up a lot of anything- is there enough for Thursday? | *Have we used up a lot of anything- is there enough for Thursday? | ||
+ | |||
+ | =====Things we need for tomorrow (Thursday):===== | ||
+ | '''General''' | ||
#Sterile tubes | #Sterile tubes | ||
#Pipette tips | #Pipette tips | ||
Line 25: | Line 32: | ||
- | Culture | + | ''''Culture' Step (from making competent cells protocol)''' |
#Sterile flasks for culture (500ml) | #Sterile flasks for culture (500ml) | ||
#Spectrophotometer and cuvettes (where) | #Spectrophotometer and cuvettes (where) | ||
Line 31: | Line 38: | ||
- | Harvest cells | + | '''Harvest cells (from making competent cells protocol)''' |
#Centrifuge/ centrifuge vial (200ml?) | #Centrifuge/ centrifuge vial (200ml?) | ||
#Ice bucket and ice | #Ice bucket and ice |
Revision as of 01:33, 18 September 2009
Lab session: 1st July
Today's introductory lab session saw the team progress with the next steps in the making competent cells procedure (i.e. steps 2 and 3 in the 'Pre-culture section). The team also looked at the future steps involved in the protocol and prepared solutions and equipment accordingly. This lab session (as well as the previous one) taught the team the importance of planning, organisation and time management.
Things needed for today's session
Things we need for today include:
- LB
- Containers
- Sterile tubes
- Space in orbital shaking incubator for tonight
Progressing with making competent cells
- We plated out single cultures from yesterday's plates for a practice.
- We inoculated 2ml of LB with single cultures from yesterday's plates and put them in the shaking incubator.
- We made up some 50% glycerol and it was sent to be autoclaved.
Preparations for next lab session
Considerations
- Have we used up a lot of anything- is there enough for Thursday?
Things we need for tomorrow (Thursday):
General
- Sterile tubes
- Pipette tips
- Solutions
'Culture' Step (from making competent cells protocol)
- Sterile flasks for culture (500ml)
- Spectrophotometer and cuvettes (where)
- Arrange space in shaking incubator
Harvest cells (from making competent cells protocol)
- Centrifuge/ centrifuge vial (200ml?)
- Ice bucket and ice
- Glycerol
- Sterile microfuge tubes
- Liquid nitrogen
News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net