Team:Newcastle/IntroductoryLabwork/1 July 2009

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Lab session: 1st July

Today's introductory lab session saw the team progress with the next steps in the making competent cells procedure (i.e. steps 2 and 3 in the 'Pre-culture section). The team also looked at the future steps involved in the protocol and prepared solutions and equipment accordingly. This lab session (as well as the previous one) taught the team the importance of planning, organisation and time management.

Things needed for today's session

Things we need for today include:

• LB
• Containers
• Sterile tubes
• Space in orbital shaking incubator for tonight

Progressing with making competent cells

• We plated out single cultures from yesterday's plates for a practice.
• We inoculated 2ml of LB with single cultures from yesterday's plates and put them in the shaking incubator.
• We made up some 50% glycerol and it was sent to be autoclaved.

Preparations for next lab session

Considerations

• Have we used up a lot of anything- is there enough for Thursday?

Things we need for tomorrow (Thursday):

General

1. Sterile tubes
2. Pipette tips
3. Solutions

'Culture' Step (from making competent cells protocol)

1. Sterile flasks for culture (500ml)
2. Spectrophotometer and cuvettes (where)
3. Arrange space in shaking incubator

Harvest cells (from making competent cells protocol)

1. Centrifuge/ centrifuge vial (200ml?)
2. Ice bucket and ice
3. Glycerol
4. Sterile microfuge tubes
5. Liquid nitrogen