Team:Newcastle/IntroductoryLabwork/1 July 2009

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Revision as of 01:36, 18 September 2009

Meetings Calendar

Lab session: 1st July

Jess streaking some agar plates

Jane applying aseptic technique

Today's introductory lab session saw the team progress with the next steps in the making competent cells procedure (i.e. steps 2 and 3 in the 'Pre-culture section). The team also looked at the future steps involved in the protocol and prepared solutions and equipment accordingly. This lab session (as well as the previous one) taught the team the importance of planning, organisation and time management.

Things needed for today's session

Things we need for today include:

LB
Containers
Sterile tubes
Space in orbital shaking incubator for tonight

Progressing with making competent cells

We plated out single cultures from yesterday's plates for a practice.
We inoculated 2ml of LB with single cultures from yesterday's plates and put them in the shaking incubator.
We made up some 50% glycerol and it was sent to be autoclaved.

Preparations for next lab session

Considerations

Have we used up a lot of anything- is there enough for Thursday?

Things we need for tomorrow (Thursday):

General

Sterile tubes
Pipette tips
Solutions

'Culture' Step (from making competent cells protocol)

Sterile flasks for culture (500ml)
Spectrophotometer and cuvettes (where)
Arrange space in shaking incubator

'Harvest cells' Step (from making competent cells protocol)

Centrifuge/ centrifuge vial (200ml?)
Ice bucket and ice
Glycerol
Sterile microfuge tubes
Liquid nitrogen

20 – 21 June 2009 - Europe workshop (London)
23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
23 October Practice Presentation (Newcastle)
23 October T-shirts are ready
27 October Practice Presentation (Sunderland)
27 October Poster is ready
30 October – 2 November 2009 - Jamboree (Boston)

@@ Line 32: / Line 32: @@
 #Solutions
 <br>
-<u>'Culture' Step</u> (from making competent cells protocol)
+<u>'Culture' Step</u> (from [https://2009.igem.org/Team:Newcastle/Project/Labwork/PhilsProtocols#Preparing_Competent_E.coli_cells_for_Heat_Shock making competent cells protocol])
 #Sterile flasks for culture (500ml)
 #Spectrophotometer and cuvettes (where)
 #Arrange space in shaking incubator
 <br>
-<u>Harvest cells</u> (from making competent cells protocol)
+<u>'Harvest cells' Step</u> (from [https://2009.igem.org/Team:Newcastle/Project/Labwork/PhilsProtocols#Preparing_Competent_E.coli_cells_for_Heat_Shock making competent cells protocol])
 #Centrifuge/ centrifuge vial (200ml?)
 #Ice bucket and ice