Team:Newcastle/IntroductoryLabwork/1 July 2009

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Revision as of 01:22, 18 September 2009

Lab session: 1st July

Jess streaking some agar plates
Jane applying aseptic technique

Things we need for today:

  • LB
  • Containers
  • Sterile tubes
  • Space in incubator for tonight


  • We plated out single cultures from yesterday's plates for a practice.
  • We inoculated 2ml of LB with single cultures from yesterday's plates and put them in the shaking incubator.
  • We made up some 50% glycerol and it was sent to be autoclaved.


Things we need for tomorrow (Thursday):

  • Have we used up a lot of anything- is there enough for Thursday?
  1. Sterile tubes
  2. Pipette tips
  3. Solutions


Culture

  1. Sterile flasks for culture (500ml)
  2. Spectrophotometer and cuvettes (where)
  3. Arrange space in shaking incubator


Harvest cells

  1. Centrifuge/ centrifuge vial (200ml?)
  2. Ice bucket and ice
  3. Glycerol
  4. Sterile microfuge tubes
  5. Liquid nitrogen



News

Events

Social Net

  • Newcastle iGEM Twitter
  • [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
  • [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]
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