Team:PKU Beijing/Notebook/Protocol/PCR

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(New page: {{PKU_Beijing/Header}} {{PKU_Beijing/Sidebar_Notebook}} {{PKU_Beijing/Header2}} ==='''Protocol for PCR with EasyPfu DNA Polymerase'''=== [[Media:PKU_Protocol_for_PCR_with_EasyPfu_DNA_Poly...)
 
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[[Team:PKU_Beijing/Notebook|Notebook]] > [[Team:PKU_Beijing/Notebook/Protocol|Protocol]] > [[Team:PKU_Beijing/Notebook/Protocol/PCR|PCR with EasyPfu DNA Polymerase]]
==='''Protocol for PCR with EasyPfu DNA Polymerase'''===
==='''Protocol for PCR with EasyPfu DNA Polymerase'''===
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[[Media:PKU_Protocol_for_PCR_with_EasyPfu_DNA_Polymerase.pdf|download PDF version]]
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<html><img src="https://static.igem.org/mediawiki/2009/9/91/PKU_Adobe_Reader_Logo.jpg" width=20></html>[[Media:PKU_Protocol_for_PCR_with_EasyPfu_DNA_Polymerase.pdf|download PDF version]]
'''Preparation for Reaction Mixture'''
'''Preparation for Reaction Mixture'''

Latest revision as of 10:12, 9 October 2009

 
Notebook > Protocol > PCR with EasyPfu DNA Polymerase

Protocol for PCR with EasyPfu DNA Polymerase

download PDF version

Preparation for Reaction Mixture

ReagentFinal concentrationVolume
10X Taq buffer1X2 µl
2.5 mM dNTP mix0.25 mM2 µl
Forward Primer0.2-0.4 µM1 µl
Reverse Primer0.2-0.4 µM1 µl
EasyPfu DNA Polymerase25 units1 µl
Template DNAAs required<0.5 µg
ddH2Oto final volume

Cycling Conditions

StepTemperature/℃Time/min
Initial Denaturation945
Denaturation940.5
Primer AnnealingAs requiredAs required
Extending72As required(0.5kb/min)
Final Extending7210
Number of Cycles 32-35
Cooling Down410



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