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| - | ==Coliguard: recovering the BioBricks==
| + | {{:Team:UNICAMP-Brazil/inc_topo}} |
| | + | {{:Team:UNICAMP-Brazil/inc calendar}} |
| | + | __NOTOC__ |
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| - | Today we ressuspended the chosen BioBricks according to the standard protocol. Each undergraduated student was responsible to ressuspend a set of BioBricks. All of them were transfomed into electrocompetent E. coli (DH10B) according to protocol 3 (see protocol section). The cells were then plated in LB media containing the appropriate antibiotic.
| + | ==''' ColiGuard '''== |
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| - | '''Marcelo'''
| + | ====Recovering the BioBricks==== |
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| - | Part:BBa_B0030 - RBS.1 (strong) -- modified from R. Weiss
| + | *<p style=”text-align:justify;”>Today we resuspended 4 BioBricks:</p> |
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| - | Part:BBa_B0032 - RBS.3 (medium) -- derivative of BBa_0030
| + | BBa_K131009 |
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| - | '''Luige'''
| + | BBa_K117002 |
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| - | Part:BBa_J61100 - Ribosome Binding Site Family Member
| + | BBa_J04430 |
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| - | Part:BBa_J61107 - Ribosome Binding Site Family Member
| + | BBa_K091109 |
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| - | '''Marcos''' | + | *<p style=”text-align:justify;”>We transformed the biobricks into ''E. coli'' DH10B competent cells and plated on adecuated antibiotic plates.</p> |
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| - | Part:BBa_B0010 - T1 from E. coli rrnB
| + | ''Luige'' |
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| - | Part:BBa_B0014 - double terminator
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| - | '''Leonardo'''
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| - | Part:BBa_B0015 - double terminator (B0010-B0012)
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| - | Part:BBa_R0040 - TetR repressible promoter
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| - | Part:BBa_J23100 - constitutive promoter family member
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| - | Part:BBa_J23107 - constitutive promoter family member
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| - | '''Fabiana'''
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| - | Part:BBa_J23109 - constitutive promoter family member
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| - | Part:BBa_J31007 - tetracycline resistance protein TetA(C)
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| - | Part:BBa_J31003 - kanamycin resistance forward
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| - | Part:BBa_E0040 - green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212)
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| - | '''Victor'''
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| - | Part:BBa_E1010: **highly** engineered mutant of red fluorescent protein from Discosoma striata
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| - | Part:pSB1A3 - High copy BioBrick assembly plasmid
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| - | Part:pSB1AK3 - High copy BioBrick assembly plasmid
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| - | Part:pSB3K3 - Low to medium copy BioBrick standard vector
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| - | Part:pSB6A1 - pBR322 converted to BioBrick vector (formally BBa_I739201)
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| | {{:Team:UNICAMP-Brazil/inc_rodape}} | | {{:Team:UNICAMP-Brazil/inc_rodape}} |
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