Team:UNICAMP-Brazil/Notebooks/July 24

(Difference between revisions)

Latest revision as of 19:47, 19 October 2009

Once we sucessfully recover the F plasmid, we now start the recovery procedure for the R plasmid. We will need this plasmid purified in order to isolate the FinO and FinP sequences (both related to the inhibition of the conjugative process).
We performed minipreps from R plasmid-containing cultures, according to Protocol 2.
We then ran an agarose gel with miniprep's sample, also running a few F plasmid's sample as a band-size control.
As we did for F plasmid, we were also able to recover the R plasmid.

Gabriel, Marcelo and Victor.

@@ Line 2: / Line 2: @@
 {{:Team:UNICAMP-Brazil/inc calendar}}
-During the week from July 20th to 25th we designed all primers that we´re going to use in our ptoject. All the sequences are showed on July 25th notebook.
+__NOTOC__
+==''' ColiGuard '''==
+==== R Plasmid Minipreps ====
+* Once we sucessfully recover the F plasmid, we now start the recovery procedure for the R plasmid. We will need this plasmid purified in order to isolate the FinO and FinP sequences (both related to the inhibition of the conjugative process).
+* We performed minipreps from R plasmid-containing cultures, according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2].
+* We then ran an agarose gel with miniprep's sample, also running a few F plasmid's sample as a band-size control.
+* As we did for F plasmid, we were also able to recover the R plasmid.
+[[image:Teste_plasmideos_10072009.jpg|center]]
+''Gabriel, Marcelo and Victor.''
 {{:Team:UNICAMP-Brazil/inc_rodape}}