Team:UNICAMP-Brazil/Notebooks/October 12

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ColiGuard

finO-biofusion - transformation results

Only two colonies grew in yesterday's plates.
Although we had experienced a low efficiency in biofusion usage, we won't continue finO's biobrick construction.

Marcelo

finOP - Final Conclusion

After several unsuccessful attempts on constructing finOP's biobricks, using different strategies with different backbones, our team decided to leave aside those biobricks.
Finalizing these parts will demand a lot of time, and we intend to already send our assembled biobricks this Thursday.
It's quite possible to fully assemble them, but we haven't enough time right now. We got a lot of work to do related on characterizing our assembled biobricks and updating this wiki you are currently reading on.
Thus, we will now focus on other kind of work. We are done with finOP inhibition system.

Marcelo

YeastGuard

pADH1+YFP (new biobrick)

Today we made the miniprep and the digestion of the plasmids containing our new briobrick (pADH1+YFP). The enzymes used were XbaI and PstI. Then, we performed the purification of the bands correspondig to the size of this new biobrick pADH1+YFP. So, it was sent to IGEM organization. =)

Wesley and Sula

@@ Line 31: / Line 31: @@
 ====pADH1+YFP (new biobrick)====
-Today we made the miniprep and the digestion of the plasmids containing our new briobrick (pDHA1+YFP). The enzymes used were XbaI and PstI.
+Today we made the miniprep and the digestion of the plasmids containing our new briobrick (pADH1+YFP). The enzymes used were XbaI and PstI.
 Then, we performed the purification of the bands correspondig to the size of this new biobrick pADH1+YFP. So, it was sent to IGEM organization. =)
+[[Image:Biobrick enviado.JPG|center]]