Team:UNICAMP-Brazil/Notebooks/October 12

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ColiGuard

finO-biofusion - transformation results

  • Only two colonies grew in yesterday's plates.
  • Although we had experienced a low efficiency in biofusion usage, we won't continue finO's biobrick construction.

Marcelo


finOP - Final Conclusion

  • After several unsuccessful attempts on constructing finOP's biobricks, using different strategies with different backbones, our team decided to leave aside those biobricks.
  • Finalizing these parts will demand a lot of time, and we intend to already send our assembled biobricks this Thursday.
  • It's quite possible to fully assemble them, but we haven't enough time right now. We got a lot of work to do related on characterizing our assembled biobricks and updating this wiki you are currently reading on.
  • Thus, we will now focus on other kind of work. We are done with finOP inhibition system.

Marcelo


YeastGuard

pADH1+YFP (new biobrick)

Today we made the miniprep and the digestion of the plasmids containing our new briobrick (pADH1+YFP). The enzymes used were XbaI and PstI. Then, we performed the purification of the bands correspondig to the size of this new biobrick pADH1+YFP. So, it was sent to IGEM organization. =)

Biobrick enviado.JPG


Wesley and Sula