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Team:UNICAMP-Brazil/Notebooks/September 30
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(New page: {{:Team:UNICAMP-Brazil/inc_topo}} {{:Team:UNICAMP-Brazil/inc calendar}} __NOTOC__ ==''' YeastGuard '''== ====Screening - new biobricks==== - This morning we found some ''E. coli'' colo...) |
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==''' YeastGuard '''== | ==''' YeastGuard '''== | ||
| - | ==== | + | ====New biobricks - screening==== |
| - | - This morning we found some ''E. coli'' colonies that were transformed yesterday! We performed PCR with 10 colonies from each biobrick (pJEN1, pDLD and | + | - This morning we found some ''E. coli'' colonies that were transformed yesterday! We performed PCR with 10 colonies from each biobrick (pJEN1, pDLD and Lysozyme) with the vector's primers (VR and VF). |
- The figure shows that unfortunately none of the colonies tested had the insert into the vector. The size of the amplicons correspond to the vector's fragment without any insert (+/- 1400bp). | - The figure shows that unfortunately none of the colonies tested had the insert into the vector. The size of the amplicons correspond to the vector's fragment without any insert (+/- 1400bp). | ||
Revision as of 12:59, 2 October 2009
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