Team:UNICAMP-Brazil/Notebooks/September 4

From 2009.igem.org

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====Electrocompetent ''E. coli''====
====Electrocompetent ''E. coli''====
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Today we prepared electrocompetent ''E. coli'' to use in our transformations according to Protocol 4 (see protocols section).
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*Today we prepared electrocompetent ''E. coli'' to use in our transformations according to Protocol 4 (see protocols section).
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==''' ColiGuards '''==  
==''' ColiGuards '''==  
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==== Cre-Recombinase without ATG ====
==== Cre-Recombinase without ATG ====
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Today we started the construction of Cre-Recombinase without ATG, in order to made a new biobrick involving this construction.  We ressuspended the biobrick of Cre-Recombinase: BBa_J61047  and transformed in E. coli DH10B eletrocompetent cells. We then plated on LB Amp plate, according to information from registry page.
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*Today we started the construction of Cre-Recombinase without ATG, in order to made a new biobrick involving this construction.  We ressuspended the biobrick of Cre-Recombinase: BBa_J61047  and transformed in E. coli DH10B eletrocompetent cells. We then plated on LB Amp plate, according to information from registry page.
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Víctor  
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''Víctor''
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Revision as of 20:41, 26 September 2009

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MicroGuards

Electrocompetent E. coli

  • Today we prepared electrocompetent E. coli to use in our transformations according to Protocol 4 (see protocols section).


ColiGuards

Cre-Recombinase without ATG

  • Today we started the construction of Cre-Recombinase without ATG, in order to made a new biobrick involving this construction. We ressuspended the biobrick of Cre-Recombinase: BBa_J61047 and transformed in E. coli DH10B eletrocompetent cells. We then plated on LB Amp plate, according to information from registry page.

Víctor