==Monday 20 July 2009==
===Results===
* Results of transformation with pSB4C5 backbone in NEB 10 beta three days after transformation
** Control (+) on LB NEB 10 beta (not shocked) had Lawn growth
** Control (-) on Cm NEB 10 beta (not shocked) had No growth
{| cellpadding="10" align="center" border="0.9" padding="5"
|+
|-
! Promoter!! Reporter !! Resistance/Plate !! Growth Observation !!
|-
! J23110
| E0240 || Cm || 62 colonies ||
|-
! J23110
| J06702 || Cm || 56 colonies ||
|-
! J23113
| E0240 || Cm || 75 colonies ||
|-
! J23113
| J06702 || Cm || 33 colonies ||
|-
! J23116
| E0240 || Cm || 41 colonies ||
|-
! J23116
| J06702 || Cm || 65 colonies ||
|-
! R0011
| E0240 || Cm || hundreds of colonies ||
|-
! R0011
| J06702 || Cm || 5 colonies ||
|-
! R0040
| E0240 || Cm || 20 colonies ||
|-
! R0040
| J06702 || Cm || 12 colonies ||
|-
! J23105
| E0240 || Cm || 26 colonies ||
|-
! J23101
| E0240 || Cm || 34 colonies ||
|}
[[User:Trentay|Trentay]] 14:17, 20 July 2009 (UTC)
''Craig and Clay''
* The registry did confirm that the correct resistance for the limonene synthesis related parts is ampicillin. New parts will also be sent.
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===Tasks===
''Maria and Afton''
* Pick colonies for overnight culture from transformations
* Ligate J23106 and J23101 with backbone pSB4C5 and RET E0240
* Transform liagated parts into NEB 10 beta
[[User:Trentay|Trentay]] 18:40, 20 July 2009 (UTC)
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====Wetlab====
*1
*2
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