EPF-Lausanne/13 October 2009
From 2009.igem.org
Contents |
Wet Lab
Clone LOVTAP into an iGEM plasmid:
LOVTAP = purified PCR product from 12 Oct 09 ( PCR on plasmid with LOVTAP-Term)
Plasmid = pSB1A3 ( Ampicilin resistance)
Digestion (both vector and insert with EcoRI and SpeI)
Checked digestion product with agarose gel
Purification
Ligation ( 1h30 )
Transformation on LB agar plate with ampicilin for selection (+did negative control)
People in the lab
Gabriela, Heidi, Tú
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