EPF-Lausanne/14 July 2009
From 2009.igem.org
Wet Lab
The digestion of the [http://partsregistry.org/Part:BBa_B0010 Terminator (BBa_B0010)] was done, in order to purify it once more, using gel extraction.
Miniprep of LacI-RBS and Inverter TetR, and PCR of the two of them.
The linearized [http://partsregistry.org/Part:BBa_B0010 Terminator (BBa_B0010)] was runned on an agarose gel and purified with a gel extraction kit.
Even though linearized [http://partsregistry.org/Part:BBa_B0010 Terminator (BBa_B0010)] concentration was very low, we tried to ligate the previously amplified LOVTAP (08.07.09) in linearized [http://partsregistry.org/Part:BBa_B0010 Terminator (BBa_B0010)]
Cloning Strategy
Primers designed for LOVTAP read-out and RBphP project.
People in the lab
- Heidi, Tu, Nath, Caro
![](https://static.igem.org/mediawiki/2009/thumb/6/61/Flèche_gauche.png/70px-Flèche_gauche.png)
![](https://static.igem.org/mediawiki/2009/thumb/5/5e/Fleche_droite.png/70px-Fleche_droite.png)