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EPF-Lausanne/20 July 2009
From 2009.igem.org
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Wet Lab
As the transformation of Saturday didn't work, we think that the plasmid Term BBa_B0010 has a problem. We tried to begin all over with 4 other Terminator from the registery.
We first did an extraction of 4 new Term plasmid from kit plate. Then we did a transformation of these 4 Term + a transf. with death cassette (with the special protocole for One Shot ccdB survival cells).
At the same time, we did a PCR with the 4 plasmids from the kit + LovTAP. We purified these PCR products, in the idea to do a digestion and then a ligation with them.
In parallel, we ran a gel with the Term plasmids, but there were no bands on the gel, so we didn't do the digestion. We will wait to have the transformation products to do that.
We also prepared LB-plates : Amp-Kana, Amp-Kana-Chl and Kana-Chl.
People in the lab
- Nathalie, Caroline, Mélanie, Tu, Heidi
