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Team:Brown/Notebook Meetings/5-4-09
From 2009.igem.org
iGEM 2009 Meeting
May 4, 2009
- 6:13PM: Meeting starts in SFH Auditorium. Discussed War War Ko’s name.
- 6:14: Project ideas. Adding Minoo’s “Pro Bread” to the list
- Yeast that makes amino acids (I, L, V) and packages
- 6:25: Pulling out E. Colirs
- 6:29: Stephanie suggests T shirts
- 6:31: https://2009.igem.org/Team:Brown
- This is the iGEM Team Wiki that we can customize
- 6:37: Tick and Treat
- Need to find out what histamine does after it binds to a histamine receptor
- EV131 HBP is a potential part to submit
- Addressed the liver targeting protein issue – need to control this to make sure it grabs a histamine before it is uptaked by liver
- Delivery system
- Timeline?
- 7:01: Safe Cell
- Will – “This is how we can prevent government regulation”
- Implementation: RFC biobrick – as part of the standard for making a device, you would have to have these genes included.
- Applicable to prokaryotes and eukayotes
- Media vs gel – we can put the repressor in the gel
- Refine holin slide
- Transcriptional repressor implementation?
- Indu will make it asthetically pleasing
- 7:21: Pro Bread
- Background is good but we should get to the point quicker
- Issues: do yeast have all the enzymes to make the essential amino acids?
- Yes. If so, then we can upregulate these enzymes
- Can we produce gram quantities?
- Will depleting pyruvate affect cells ability to make amino acids because we are depleting some of its ATP source
- Can we FIND the rate limiting step of yeast ILV biosynthesis pathways? Upregulating this step will produce more amino acids
- Negative feedback – can we prevent this? Packaging?
- 7:54: Eli’s Cog Sci Project
- 7:58: Histamine: Indu, Eli, Ashley
- Safe Cell: Ahmad, Michael, Flora
- Pro Bread: Minoo, Will, Steph
- 7:59: NEXT MEETING: Thursday afternoon 3:00 PM
- BRING YOUR FINAL POWERPOINTS!! And make the changes.
