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Team:Brown/Notebook Meetings/5-6-09
From 2009.igem.org
iGEM 2009 Meeting
May 6, 2009
- 5:09: Meeting begins in SFH 218, with Adrian and Dana. Today we are presenting our powerpoints for Friday’s panel meeting
- 5:09: Minoo: engineering yeast used to make breast to upregulate production 3 essential amino acid
- A: LEU3 promotes transcription of genes that produces more amino acids
- B: Increase production of pyruvate: amylase breaks down starch to produce more pyruvate; genes for this have already been made
- C: packaging and secretion by ILVp gene – package into ILV rich proteins
- Adrian: why don’t we find an endogenous protein in yeast that has high ILV content
- Steal some pathways from Rice 2007
- 5:29: Indu: solving allergies
- Pro biotic – can we induce the protein secretion into the blood?
- 5:59: CCDB – 3rd mechanism poisons DNA gyrase
- It’s in the registry
- Leaky promoters ? issue of cell dying when you want it to live
- Find balance where expression of UCP is enough and where leakiness doesn’t affect cells
- Selective pressure to keep the plasmid
- If the UCP is not made then it will be mutated and not work
