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Team:Groningen/Notebook/18 September 2009
From 2009.igem.org
[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]
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Wet
GVP Cluster
Planning
- → TODO work out the wiki page for GVP
- made a layout
- still have to read all articles
- modeling will stay as it is, has been done by modeling people
- → DONE make a doodle for presentation planning (1-19 oct.)
- → DONE media attention
- mail to UK, Ing., St. Gen.
- facebook account with link to twitter
- ethics survey link on facebook and twitter
- → DONE place an ethics survey link on twitter
- → DONE clone pArsR-GVP into pSB2K3
- TODO still need to make glycerol stock
- → TODO clone repeat out of GVP cluster
- → TODO make glycerol stocks of constructs
- → TODO enter info on part registry
Plates
| Name | Plasmid Used | Antibiotics on Plasmid | No. of Colonies | Date |
| Positive (high) | J61002 (with J23101) | Ampicillin | ~1500 (red) | 17 sept. |
| Negative (high) | None | None | 8 (white/yellow) | 17 sept. |
| pMA-gvpL (low) | pMA | Ampicillin | ~150 | 17 sept. |
| pMA-gvpL (high) | pMA | Ampicillin | ~650 | 17 sept. |
| pMA-GlpF (low) | pMA | Ampicillin | ~125 | 17 sept. |
| pMA-gvpL (high) | pMA | Ampicillin | ~550 | 17 sept. |
| pNL29 (SJ)(low) | pBlueScript_II_KS_(+) | Ampicillin | ~100 | 17 sept. |
| pNL29 (high) | pBlueScript_II_KS_(+) | Ampicillin | ~250 | 17 sept. |
| pArsR-GVP (on Ampicillin) | pSB2K3 | Kanamycin | 0 | 17 sept. |
| pArsR-GVP (Glycerol Stock) | pSB2K3 | Kanamycin | Single Colonies | 17 sept. |
| pArsR-GVP (Saline Test) | pSB2K3 | Kanamycin | Plate Full-Grown | 17 sept. |
| pZntR-GVP (Saline Test) | pSB2K3 | Kanamycin | Plate Full-Grown | 17 sept. |
| pCueO-GVP (Saline Test) | pSB2K3 | Kanamycin | Plate Full-Grown | 17 sept. |
- → The plates showed the expected amount of colonies, and also red in the case of the positive plates. The eight colonies on the negative plate can be taken into acount when plating transformed cells.
- → The plate with stripes of pArsR-GVP (pSB2K3) preculture showed growth and some single colonies.
- → All plates were stored in the fridge for further use on monday.
Cultures
The overnight cultures with LB-amp100 medium of colonies E.coli TOP10 with pNL29 in pBlueScript_II_KS_(+) showed no growth.
- → The plate used for culture inoculation was very (more than a month) old, and was probably no good anymore. The new transformed E.coli TOP10 with original pNL29 plasmid will replace all excisting stocks/plates.
Transporters
Metal Accumulation
Vectors
Dry
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