Team:Newcastle/Meetings/6 August 2009

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Contents 1 6 August 2009 - iGEM Meeting 1.1 Agenda 1.2 Action Points during last meeting 1.3 New action points 1.4 Minutes

6 August 2009 - iGEM Meeting

• Chair: James
• Students:
• Minutes: Goksel
• Instructors:
• Advisors:

Agenda

• Lab Summary
• Sequencher usage
  • Protocol for recovering sleB/cwlJ, given one is for cwlD only.
  • Do we need another RBS between GFP and KinA (multiple CDS).
  • Do we need GFP in the biobrick design at all? (SpoTeam)
• Review last week's action points
• Any questions

Action Points during last meeting

• Ask about project description (Jess) - Done.
• Send BioBrick design to Neil (All).
• Ask Pr.Nigel Robinson if he wants to be an advisor (Mat) - Pending.
• Think about the project title; BacMan,the Quest to Sequester? - Morgan likes, accepted.
• Need to put more pictures on the wiki (Mat).
• Collect pictures of the team from those who took pictures (Arun).
• Write personal descriptions on the wiki and ask the instructors and advisor to do so (Arun) - Part done.
• Have a look at the shuttle vector that Cambridge submitted last year (Leave for later).
• Look at Groningen's BioBrick design (leave for later).
• Need to set up a meeting with Noel from Sunderland (Goksel).
• Put modelling stuff and presentations on the wiki (Mat).
• Need to calculate how much the stochastic BioBrick will cost (Jess).
• The need for more money (Sponsors) - Arun and Mat emailed.
  • Are Millipore still a sponsor? - Arun emailed.
• Lab PC still needs drivers installed - students account not admin.
• T-shirts to be designed.

New action points

• Design biobricks and primers in Sequencher.
• Submit Preliminary Project Track details to iGEM HQ by 7 August (TOMORROW!).
• Change NheI cutsite with another cutsite in teh designs since it is used in the integration vectors
• Find the integration vector to use at the lab
• Find out for which nongerminating strains is the protocol
• Use pmutin4's promoter for the pspac promoter in the designs
• Do not knock out the original cotC in Bacillus subtilis strain
• Write to the Cornell team for collaboration.
• Contact to Groningen team (Neil)

Minutes

• We have been sent two strains in spore forms and they cannot germinate. In one of them, the spore coat is engineered and in the other one enzymes requierd the break the spore wall are not produced to prevent germination.
• We can run teh primers on 2% ararose gel.
• Primer synthesis is a quick and cheap process
• We may use lacZ for the promoter library