Team:UQ-Australia/Notebook/Experiment2

From 2009.igem.org

Experiment 2

Transformation of pSUTp. pG.pmt and pSUTp

The bacteria used for this transformation reaction was DH5α Max efficiency cells strain (chemical competent) pSUTp. pG. pmt and pSUTp solution collected from 02/10/09 (click HERE fore further details) (Can you please put the link to 02/10.09 nanodrop result here?) were diluted to 10ng/uL solution to use for the transformation reaction.

Transformation control: pUC19

  • Click HERE for detailed protocol of transformation reaction.
  • While bacteria containing pSUTp.pG.pmt and pUC 19 were cultured on two separate Amp plates, bacteria with pSUTp was grown on Kan plate.
  • Incubate went overnight at 370C.
  • Results: Bacteria colonies were shown on plate in following morning
  • 08/10/09: Pick isolate colonies from pSUTp and pSUTp.pG.pmt cultures.

Click HERE for detailed protocol of picking colonies.

  • 4 colonies from pSUTp culture were picked and transfered into new tubes named pSUTp Kan 1-4
  • 4 colonies from pSUTp.pG.pmt were picked and transferred into new tubes named pSUTp.pG.pmt Amp 1-4

NOTE: MerT and MerP primers arrived.

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