Team:Warsaw/Calendar-Main/22 August 2009
From 2009.igem.org
Assembly of endosomal detection operon
Marcin
Task 1:
- Electrophoresis of plasmid isolated and subsequently digested in: 21.08.09
Results:
All samples of cro CDS on pSB1A3 are without insert (samples 1-8)
All ligations of p53 CDS were correct (samples A-D)
All ligations of p53 CDS were correct (samples A-D)
Comment:
The most probable explanation of this situation is circularization of empty plasmid because I have forgotten to dephosphorylate vector using CIAP
Task 2:
- Prepare the bacterial cultures for isolation of following construct: BBa_C0040 with BBa_B0032 + BBa_R0080 on pSB1A3 plasmid
Making BioBricks' stocks
Justyna
Task:
- Making the glycerol stocks of bacteria bearing BioBricks.
B0032+C0040, C0051+B0032, E0032+B0032, E0022+BOO32, P53+pSB1A3.
Making of the plac-RBS-llo-intA part
Jarek
Tasks:
- Digestion of the the plac+RBS+llo and internalin A with PstI endonuclease.
- Separation of digestion products in 0,8% agarose gel.
- Cuting the agarose blocks containing the digested DNA from gel.
- Preparation of pure DNA samples from agarose blocks with A&A GelOut kit.
- Preparation of ligation containing digested internalin A and vector containing plac+RBS+llo part.
Ania
Tasks:
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