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Team:Warsaw/Notebook/toc
From 2009.igem.org
Table of Contents
(or short description of what are we trying to do)
1. Cloning of natural bricks:
- PhoP/PhoQ operon + mgtc promoter
- Mitochondrium-targeting sequence from the COX gene
- Secretion system from Escherichia coli
- Bricks responsible for invasiveness:
- Listeriolysin from Listeria monocytogenes
- Internalin A from Listeria monocytogenes
- Invasin from Yersinia sp.
- Apoptotic proteins:
- P53 (directed to mitochondrium)
- Bax
2. Assembly of bricks:
- Available bricks + RBS
- Assembly of operon parts
- Assembly of testing devices
3. Testing of prepared bricks/devices:
- EMSA on cro/cro-box
