Uppsala-Sweden/6 August 2009

From 2009.igem.org




Plasmid Prep for PpetE

Plasmid prep was performed using [http://www.sigmaaldrich.com/life-science/molecular-biology/dna-and-rna-purification/plasmid-miniprep-kit.html GenElute™ Plasmid Miniprep Kit]


Digestion of ADH2(Z),pirB, pdc, PpetE, adh2(Y)

Digestion was performed using EcoRI and PstI from Fermentas with this protocol ([http://www.fermentas.com/fastdigest/index.html Protocol for Fast Digestion of Unpurified PCR Products with FastDigest® Enzymes)])

The calculated amounts for the digestion are written down here : Media:Digestion_06082009.xls


Purification of ADH2(Z),pirB, pdc, PpetE, adh2(Y)

Purification of the digestion mix was performed with [http://www.clontech.com/images/pt/PT3814-1.pdf Nucleo Spin® Extract 2], PCR cleanup protocol from Clontech

Purified product (50µl) in 1.5ml eppendorfs.


--Karl.brune 08:56, 10 August 2009 (UTC)

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