Team:UNICAMP-Brazil/Notebooks/September 14

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==''' ColiGuard '''==
==''' ColiGuard '''==
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====Culture growth of bacteria containing the F plasmid====
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*<p style=”text-align:justify;”>From a permanent culture of bacteria containig the F plasmid, a sample was innoculated into liquid LB-AMP medium and let grown O/N at 37°C.</p>
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''Gabriel''
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====finO and finP's transformed bacteria====
====finO and finP's transformed bacteria====

Revision as of 19:00, 17 October 2009

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ColiGuard

Culture growth of bacteria containing the F plasmid

  • From a permanent culture of bacteria containig the F plasmid, a sample was innoculated into liquid LB-AMP medium and let grown O/N at 37°C.

Gabriel


finO and finP's transformed bacteria

  • All of yesterday's transformed and plated cells grew in the media!

  • We then selected 10 colonies of each transformation (finO+pSB1A3 and finP+pSB1A3) in order to confirm them. We inoculated those colonies separately into liquid LB-AMP medium, and let then grow O/N at 37ºC.

  • We will then proceed to the minipreps of the cultures and, once we could make another PCR for the inserted fragments, we will be able to confirm that both finO and finP are indeed in biobrick format.

Marcelo






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