Team:PKU Beijing/Parts Characterization/BBa K228004

From 2009.igem.org

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[[Team:PKU_Beijing/Parts|Parts]] > [[Team:PKU_Beijing/Parts_Characterization|Parts Characterization]] > [[Team:PKU_Beijing/Parts_Characterization/BBa_K228004|BBa_K228004]]
[[Team:PKU_Beijing/Parts|Parts]] > [[Team:PKU_Beijing/Parts_Characterization|Parts Characterization]] > [[Team:PKU_Beijing/Parts_Characterization/BBa_K228004|BBa_K228004]]
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==='''BBa K228004'''===
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==='''BBa K228004 - NahR( reverse) - salicylate promoter '''===
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<partinfo>BBa_K228004 short</partinfo>
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Designed by Lin Min  Group: iGEM09_PKU_Beijing  (2009-09-18)<br>
Designed by Lin Min  Group: iGEM09_PKU_Beijing  (2009-09-18)<br>
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=='''Description'''==
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==='''Description'''===
This is a salicylate inducible promoter part constructed by Lin Min. The activator protein (NahR) gene is in the upstream but in an opposite direction of the regulated promoter. In the presence of salicylate, the promoter can be activated. If we place a generator on its downstream, the transcription of the coding sequence will be triggered by the salicylate-binded positive transcriptional activator protein which is encoded by NahR.  
This is a salicylate inducible promoter part constructed by Lin Min. The activator protein (NahR) gene is in the upstream but in an opposite direction of the regulated promoter. In the presence of salicylate, the promoter can be activated. If we place a generator on its downstream, the transcription of the coding sequence will be triggered by the salicylate-binded positive transcriptional activator protein which is encoded by NahR.  
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For the purpose of characterization, we connected [http://partsregistry.org/Part:BBa_E0840 BBa_E0840] downstream of the salicylate promoter to make the composite part, [http://partsregistry.org/Part:BBa_K228850 BBa_K228850]. This report system allowed us to test the salicylate inducible promoter indirectly via the fluorescence output of [http://partsregistry.org/Part:BBa_E0840 BBa_E0840], while the input is salicylate solution at a gradient of concentration.  
For the purpose of characterization, we connected [http://partsregistry.org/Part:BBa_E0840 BBa_E0840] downstream of the salicylate promoter to make the composite part, [http://partsregistry.org/Part:BBa_K228850 BBa_K228850]. This report system allowed us to test the salicylate inducible promoter indirectly via the fluorescence output of [http://partsregistry.org/Part:BBa_E0840 BBa_E0840], while the input is salicylate solution at a gradient of concentration.  
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=='''Performance'''==
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==='''Performance'''===
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{|cellpadding=3
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Measured by Haoqian Zhang & Chengzhe Tian, 2009
Measured by Haoqian Zhang & Chengzhe Tian, 2009
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=='''Compatibility'''==
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==='''Compatibility'''===
'''Chassis''': Device has been shown to work in DH5α.
'''Chassis''': Device has been shown to work in DH5α.
'''Plasmids''': Device has been shown to work on pSB1A2 and pSB1A3, pSB4K5
'''Plasmids''': Device has been shown to work on pSB1A2 and pSB1A3, pSB4K5
'''Devices''': Device has been shown to work with [http://partsregistry.org/Part:BBa_K228013 BBa_K228013], [http://partsregistry.org/Part:BBa_K228227 BBa_K228227] - [[Part:BBa_K228235|BBa_K228235]], [http://partsregistry.org/Part:BBa_K228255 BBa_K228255] - [http://partsregistry.org/Part:BBa_K228263 BBa_K228263], [http://partsregistry.org/Part:BBa_K228851 BBa_K228851], [http://partsregistry.org/Part:BBa_K228852 BBa_K228852] - [http://partsregistry.org/Part:BBa_K228860 BBa_K228860], [http://partsregistry.org/Part:BBa_K228870 BBa_K228870] - [http://partsregistry.org/Part:BBa_K228878 BBa_K228878].  
'''Devices''': Device has been shown to work with [http://partsregistry.org/Part:BBa_K228013 BBa_K228013], [http://partsregistry.org/Part:BBa_K228227 BBa_K228227] - [[Part:BBa_K228235|BBa_K228235]], [http://partsregistry.org/Part:BBa_K228255 BBa_K228255] - [http://partsregistry.org/Part:BBa_K228263 BBa_K228263], [http://partsregistry.org/Part:BBa_K228851 BBa_K228851], [http://partsregistry.org/Part:BBa_K228852 BBa_K228852] - [http://partsregistry.org/Part:BBa_K228860 BBa_K228860], [http://partsregistry.org/Part:BBa_K228870 BBa_K228870] - [http://partsregistry.org/Part:BBa_K228878 BBa_K228878].  
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<!-- Uncomment this to enable Functional Parameter display
 
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===Functional Parameters===
 
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<partinfo>BBa_K228004 parameters</partinfo>
 
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<!-- -->
 
{{PKU_Beijing/Foot}}
{{PKU_Beijing/Foot}}
__NOTOC__
__NOTOC__

Revision as of 00:43, 18 October 2009
 
Parts > Parts Characterization > BBa_K228004

BBa K228004 - NahR( reverse) - salicylate promoter

Designed by Lin Min Group: iGEM09_PKU_Beijing (2009-09-18)
Input: Salicylate molecules
Output: GFP fluorescence

Part Main Page Transfer Function Growth Rate

Description

This is a salicylate inducible promoter part constructed by Lin Min. The activator protein (NahR) gene is in the upstream but in an opposite direction of the regulated promoter. In the presence of salicylate, the promoter can be activated. If we place a generator on its downstream, the transcription of the coding sequence will be triggered by the salicylate-binded positive transcriptional activator protein which is encoded by NahR.

It is necessary to notice that there is no terminator downstream of the NahR coding sequence, but it has no effect son parts that are in the same direction as the pSal promoter. However, if coding gene is placed upstream of this part and in the same direction as NahR, it may cause unexpected expression.

For the purpose of characterization, we connected [http://partsregistry.org/Part:BBa_E0840 BBa_E0840] downstream of the salicylate promoter to make the composite part, [http://partsregistry.org/Part:BBa_K228850 BBa_K228850]. This report system allowed us to test the salicylate inducible promoter indirectly via the fluorescence output of [http://partsregistry.org/Part:BBa_E0840 BBa_E0840], while the input is salicylate solution at a gradient of concentration.

Performance

Experiment*Characteristics*Value & Result*
Transfer Function Maximum Output

(Output tested in a time sequence)

[http://partsregistry.org/Part:BBa_K228004:Transfer#data2 Click here]
Hill coefficient

(Output tested in a time sequence)

[http://partsregistry.org/Part:BBa_K228004:Transfer#data2 Click here]
Switch Point

(Output tested in a time sequence)

[http://partsregistry.org/Part:BBa_K228004:Transfer#data2 Click here]
Demand Decrease on Growth Rate

(Input at a gradient of concentration)

The decrease level has significantly positive correlation with the input, the concentration of salicylate.

Measured by Haoqian Zhang & Chengzhe Tian, 2009

Compatibility

Chassis: Device has been shown to work in DH5α. Plasmids: Device has been shown to work on pSB1A2 and pSB1A3, pSB4K5 Devices: Device has been shown to work with [http://partsregistry.org/Part:BBa_K228013 BBa_K228013], [http://partsregistry.org/Part:BBa_K228227 BBa_K228227] - BBa_K228235, [http://partsregistry.org/Part:BBa_K228255 BBa_K228255] - [http://partsregistry.org/Part:BBa_K228263 BBa_K228263], [http://partsregistry.org/Part:BBa_K228851 BBa_K228851], [http://partsregistry.org/Part:BBa_K228852 BBa_K228852] - [http://partsregistry.org/Part:BBa_K228860 BBa_K228860], [http://partsregistry.org/Part:BBa_K228870 BBa_K228870] - [http://partsregistry.org/Part:BBa_K228878 BBa_K228878].



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