Team:UNICAMP-Brazil/Notebooks/September 27

(Difference between revisions)

Revision as of 17:08, 18 October 2009

We selected 4 colonies from each plate containing one of yesterday's transformed biobricks, and inoculated them into liquid LB-AMP medium.
Those inocula were grew for an O/N period at 37ºC (250 rpm).

Marcelo

We pretend to ligate the parts which we are recovering right now (started yesterday) onto biobricks BBa_R0040 (pTet) and BBa_B0015 (double terminator), using it's own vector.
Those biobricks were already recovered on August 10th.
BBa_R0040 was digested with SpeI and PstI, once we intend to add a fragment in front of it (downstream). As for BBa_B0015, the digestion was realized with EcoRI and Xba restriction enzymes, once we need to insert a fragment behind it's part (upstream).
Digestion lasted 3 hours.

Marcelo

The E.coli didn´t grow in any of the plates. =[ We think that we didn´t plate an enough volume of bacteria. So we decided to replate a larger volume. We hope to find colonies tomorrow!

Raíssa and Taís

@@ Line 16: / Line 16: @@
 *<p style=”text-align:justify;”>We pretend to ligate the parts which we are recovering right now (started yesterday) onto biobricks BBa_R0040 (pTet) and BBa_B0015 (double terminator), using it's own vector.</p>
 *<p style=”text-align:justify;”>Those biobricks were already recovered on August 10th.</p>
-*<p style=”text-align:justify;”>BBa_R0040 was digested with SpeI and PstI, once we intend to add a fragment in front of it (downstream). As for BBa_B0010, the digestion was realized with EcoRI and Xba restriction enzymes, once we need to insert a fragment behind it's part (upstream).</p>
+*<p style=”text-align:justify;”>BBa_R0040 was digested with SpeI and PstI, once we intend to add a fragment in front of it (downstream). As for BBa_B0015, the digestion was realized with EcoRI and Xba restriction enzymes, once we need to insert a fragment behind it's part (upstream).</p>
 *<p style=”text-align:justify;”>Digestion lasted 3 hours.</p>