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Team:UNICAMP-Brazil/Notebooks/October 5
From 2009.igem.org
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(New page: {{:Team:UNICAMP-Brazil/inc_topo}} {{:Team:UNICAMP-Brazil/inc calendar}} __NOTOC__ ==''' ColiGuard '''== ====finOP and Cre-Recombinase with pGEM strategy==== * Today we performed minip...) |
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====finOP and Cre-Recombinase with pGEM strategy==== | ====finOP and Cre-Recombinase with pGEM strategy==== | ||
| - | * Today we performed minipreps from yesterday's inoculated cultures, according to | + | * Today we performed minipreps from yesterday's inoculated cultures, according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2]. |
* We expect to recover pGEM plasmids finally containing singly our inserts for finO, finP and Cre-Recombinase w/o ATG. | * We expect to recover pGEM plasmids finally containing singly our inserts for finO, finP and Cre-Recombinase w/o ATG. | ||
* As both sides of the inserts contains the A stick end, it's possible that we will find in some samples unwanted ligations (it could be ligate in an upside down position). Therefore, we will also need to perform PCRs to confirm that our inserts indeed are in the correct frame position. | * As both sides of the inserts contains the A stick end, it's possible that we will find in some samples unwanted ligations (it could be ligate in an upside down position). Therefore, we will also need to perform PCRs to confirm that our inserts indeed are in the correct frame position. | ||
Revision as of 17:42, 18 October 2009
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