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Team:Groningen/Notebook/19 June 2009
From 2009.igem.org
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(New page: {{Team:Groningen/Notebook/Day| *All o/n cultures were succesfull. *30% glycerol stocks were made, the stocks were flash frozen before they were put in -80dg freezer. (labeled with BBa_nr, ...) |
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*DNA was isolated from them, using the Nucleospin plasmid isolation kit. | *DNA was isolated from them, using the Nucleospin plasmid isolation kit. | ||
**Concentration of the samples were: | **Concentration of the samples were: | ||
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*1ug of the gvp containing plasmids were digested with EcoRI and XbaI in 2x buffer Tango, for 1hr @ 37dg. | *1ug of the gvp containing plasmids were digested with EcoRI and XbaI in 2x buffer Tango, for 1hr @ 37dg. | ||
*These samples and the uncut plasmids containing gvp, RBS and Term were loaded on an Agarose gel. | *These samples and the uncut plasmids containing gvp, RBS and Term were loaded on an Agarose gel. | ||
*For gvp/EcoRI-XbaI a band of 1.4kb and >5kb was found. This correlates to the expected size. | *For gvp/EcoRI-XbaI a band of 1.4kb and >5kb was found. This correlates to the expected size. | ||
| - | *For the other plasmids several bands were seen. | + | *For the other plasmids several bands were seen.}} |
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Revision as of 15:43, 23 June 2009
[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]
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- All o/n cultures were succesfull.
- 30% glycerol stocks were made, the stocks were flash frozen before they were put in -80dg freezer. (labeled with BBa_nr, gene nr1-3 and date)
- DNA was isolated from them, using the Nucleospin plasmid isolation kit.
- Concentration of the samples were:
- 1ug of the gvp containing plasmids were digested with EcoRI and XbaI in 2x buffer Tango, for 1hr @ 37dg.
- These samples and the uncut plasmids containing gvp, RBS and Term were loaded on an Agarose gel.
- For gvp/EcoRI-XbaI a band of 1.4kb and >5kb was found. This correlates to the expected size.
- For the other plasmids several bands were seen.
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