Team:Groningen/Notebook/19 June 2009

From 2009.igem.org


Igemhomelogo.png
  • All o/n cultures were succesfull.
  • 30% glycerol stocks were made, the stocks were flash frozen before they were put in -80dg freezer. (labeled with BBa_nr, gene nr1-3 and date)
  • DNA was isolated from them, using the Nucleospin plasmid isolation kit.
    • Concentration of the samples were:
  • 1ug of the gvp containing plasmids were digested with EcoRI and XbaI in 2x buffer Tango, for 1hr @ 37dg.
  • These samples and the uncut plasmids containing gvp, RBS and Term were loaded on an Agarose gel.
  • For gvp/EcoRI-XbaI a band of 1.4kb and >5kb was found. This correlates to the expected size.
  • For the other plasmids several bands were seen.
Restriction19juni.jpeg


April
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30