Team:UNICAMP-Brazil/Notebooks/September 3

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*<p style=”text-align:justify;”>Today we prepared solutions, materials and the pre inoculum to prepare electrocompetent ''E. coli''.</p>
*<p style=”text-align:justify;”>Today we prepared solutions, materials and the pre inoculum to prepare electrocompetent ''E. coli''.</p>
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====Hemolysin Operon Amplification====
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*<p style=”text-align:justify;”>We did the PCR to amplify the hemolysin operon using the total DNA extration that we had made before. Our sequence have almost 4000bp so we used a extension time of 3 minutes, the annelling temperatures in Celsius degrees was 56, 54, 52 and 51. No DNA fragment was shown in the agarose gel. .</p>
==''' YeastGuard '''==
==''' YeastGuard '''==

Revision as of 13:29, 20 October 2009

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MicroGuards

Electrocompetent E. coli

  • Today we prepared solutions, materials and the pre inoculum to prepare electrocompetent E. coli.


Hemolysin Operon Amplification

  • We did the PCR to amplify the hemolysin operon using the total DNA extration that we had made before. Our sequence have almost 4000bp so we used a extension time of 3 minutes, the annelling temperatures in Celsius degrees was 56, 54, 52 and 51. No DNA fragment was shown in the agarose gel. .

YeastGuard

Terminator Biobrick

  • The terminator plasmid was quantified and we made another digestion, Protocol X (see protocols section). We found by electrophoresis that it worked!

Wesley




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