Team:UNICAMP-Brazil/Notebooks/October 4

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====Transformation of the BBa K112806 + BBa B0015 ligation====
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*<p style=”text-align:justify;”>We transformed the ligation made yesterday by eletroporation according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Electroporation  Protocol 3]</p>
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''Léo''

Revision as of 20:24, 20 October 2009

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ColiGuard

finOP and Cre-Recombinase with pGEM strategy

  • Yesterday's transformed and plated cells grew in the media! We found white and blue colonies in the plate just as expected (due to the beta-galactosidase gene contained in pGEM vector).
  • We selected 10 white colonies (those theorically contains our inserts in the correct position, since it interrupts the coding sequence of beta-galactosidase) from each plate and inoculated them into liquid LB-AMP media.
  • Inocula were keep at 37ºC, under 250 rpm, for an O/N period.

Fabi, Leo, Marcelo and Victor





Transformation of the BBa K112806 + BBa B0015 ligation

  • We transformed the ligation made yesterday by eletroporation according to the Protocol 3

Léo

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