Team:UNICAMP-Brazil/Notebooks/October 14

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(New page: {{:Team:UNICAMP-Brazil/inc_topo}} {{:Team:UNICAMP-Brazil/inc calendar}} __NOTOC__ ==''' YeastGuard '''== ====New strategy: pGEM==== *<p style=”text-align:justify;”>We did miniprep...)
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*<p style=”text-align:justify;”>We digested the Adh1+Lysozyme/biofusion minipreps in order to connect it to the yeast expression vector (YEP). We used the ''Xba''I and ''Pst''I enzymes. 9 of 10 digestions worked! =) The expected fragment size is 2000bp.</p>
*<p style=”text-align:justify;”>We digested the Adh1+Lysozyme/biofusion minipreps in order to connect it to the yeast expression vector (YEP). We used the ''Xba''I and ''Pst''I enzymes. 9 of 10 digestions worked! =) The expected fragment size is 2000bp.</p>
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GEL
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[[Image:adh1+lis.jpg|500px|center]]
   
   
*<p style=”text-align:justify;”>We chose two fragments and purified them from the agarose gel. We ligated them to YEP. We will transform it tomorrow.</p>
*<p style=”text-align:justify;”>We chose two fragments and purified them from the agarose gel. We ligated them to YEP. We will transform it tomorrow.</p>

Revision as of 04:18, 21 October 2009

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YeastGuard

New strategy: pGEM

  • We did miniprep of 10 Adh1+Lysozyme colonies.

  • We digested the Adh1+Lysozyme/biofusion minipreps in order to connect it to the yeast expression vector (YEP). We used the XbaI and PstI enzymes. 9 of 10 digestions worked! =) The expected fragment size is 2000bp.

Adh1+lis.jpg

  • We chose two fragments and purified them from the agarose gel. We ligated them to YEP. We will transform it tomorrow.


Raíssa and Taís




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