Team:UNICAMP-Brazil/Notebooks/August 21

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====Recovered biobricks====
====Recovered biobricks====
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'''Miniprep, digestion and ligation of RBS'''
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'''Miniprep and digestion of RBS'''
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* Today we perfomed miniprep from the RBS transformed ''E. coli'' according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2]. We digested the plasmids with the ''Spe''I and ''Pst''I enzymes([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Digestion_reaction Protocol 14]) and the fragment size wasn't expected =(
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* Today we perfomed miniprep from the RBS transformed ''E. coli'' according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2]. The fragment size seamed to be correct and we decided to digest it according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Digestion_reaction Protocol 14]  with the ''Spe''I and ''Pst''I enzymes. It worked.
 
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* After the RBS plasmid digestion, we ligated it to CFP previously digested with ''Xba''I and ''Pst''I according to the  [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/T4_DNA_Ligase Protocol 11].
 
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[[Image:RBSdigestion.jpg‎|500px|center]]
 
''Raíssa''
''Raíssa''
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Revision as of 06:27, 21 October 2009

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Recovered biobricks

Miniprep and digestion of RBS

  • Today we perfomed miniprep from the RBS transformed E. coli according to the Protocol 2. We digested the plasmids with the SpeI and PstI enzymes(Protocol 14) and the fragment size wasn't expected =(



Raíssa




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