Team:UNICAMP-Brazil/Notebooks/August 22

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(New page: {{:Team:UNICAMP-Brazil/inc_topo}} {{:Team:UNICAMP-Brazil/inc calendar}} __NOTOC__ ==''' YeastGuard '''== ====Recovered biobricks==== '''RBS+CFP''' * Today we perfomed transformed ''E...)
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==''' YeastGuard '''==
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====Recovered biobricks====
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'''RBS+CFP'''
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* Today we perfomed transformed ''E. coli'' according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2] with the ligation reaction of RBS+CFP. The fragment size seamed to be correct and we decided to digest it according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Digestion_reaction Protocol 14]  with the ''Spe''I and ''Pst''I enzymes. It worked.
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* After the RBS plasmid digestion, we ligated it to CFP previously digested with ''Xba''I and ''Pst''I according to the  [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/T4_DNA_Ligase Protocol 11].
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[[Image:RBSdigestion.jpg‎|500px|center]]
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''Raíssa''
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{{:Team:UNICAMP-Brazil/inc_rodape}}
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