Team:Kyoto/CiC/Notebook/0907-0911

From 2009.igem.org

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(097-0911 : Plasmid Construction of HIV-TAT-(LALAAAA)3-generator)
(Thursday, 17 September)
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===Thursday, 17 September===
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===Fryday, 11 September===
====To Do====
====To Do====
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*Make repetitive sequence (MPR)
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*Ligation
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*Transformation(retry)
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*Transformation
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Revision as of 09:50, 21 October 2009

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0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

097-0911 : Plasmid Construction of HIV-TAT-(LALAAAA)3-generator


Wednesday, 16 September

To Do

  • PCR
    • HIV-TAT-(LALAAAA)3
  • Restriction Enzyme Digestion
    • HIV-TAT-(LALAAAA)3 (EcoR1 + Spe1)
    • B0015 (EcoR1 + Spe1)
  • Electrophoresis

Results



Fryday, 11 September

To Do

  • Ligation
  • Transformation