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Team:Kyoto/CiC/Notebook/0921-0925
From 2009.igem.org
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*TIM23complex signal::EGFP generator | *TIM23complex signal::EGFP generator | ||
**miniprep PEGFP-N3 VECTOR (EGFP generator) | **miniprep PEGFP-N3 VECTOR (EGFP generator) | ||
| + | **phosphorylation signal sequence primer | ||
====Results==== | ====Results==== | ||
*消えた | *消えた | ||
Revision as of 12:31, 21 October 2009
- 0907-0911
- constructing HIV-TAT-(LALAAAA)3-generator
- 0914-0918
- constructing HIV-TAT-(LALAAAA)3-generator
- 0921-0925
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 0928-1002
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1005-1009
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1012-1016
- Observation
- 1019-1023
- Observation
0914-0918 : what to do. what to do. what to do. what to do.
Monday, 21 September
To Do
- HIV-TAT-(LALAAAA)3-generator
- miniprep
- TIM23complex signal::EGFP generator
- miniprep PEGFP-N3 VECTOR (EGFP generator)
- phosphorylation signal sequence primer
Results
- 消えた
Tuesday, 22 September
To Do
- HIV-TAT-(LALAAAA)3-generator
- miniprep
- TIM23complex signal::EGFP generator
- PEGFP-N3 VECTOR (EGFP generator)
Results
- 消えた
Wednesday, 23 September
To Do
- ligation
- transformation
Results
ligation
| NO. | Sample name | conc./(ng/ul) |
|---|---|---|
| 1 | 11(stu1) | 103.2 |
Transformation
No colony was observed (090917 10:00)
Thursday, 17 September
To Do
- Make repetitive sequence (MPR)
- Transformation(retry)
