Team:KU Seoul/Overview

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Overview

Integrated Heavy Metal Detection System

Our team project is designing synthetic modules for simultaneous detection of multiple heavy metals such as

arsenic, zinc, and cadmium in E. coli. The ultimate goal is to build a micromachine sensing and determining of

the concentration of heavy metals in a sample solution (e.g. the waste water). In order to design the system,

we will employ two fluorescence proteins (GFP and RFP) and aryl acylamidase as signal reporters. Since each

heavy metal promoter produces unique fluorescence or color by those reporters, if more than two heavy metals

coexist in a solution, the results would be interpreted from the convoluted fluorescence and/or color rather

than a single signal detection. The successful construction of the synthetic modules in E. coli can be utilized

in the form of a lyophilized powder, which can be stored in a drug capsule to make it portable.

STEP1, Prepare plasmids.

STEP2. Gene cloning & Infusion Biobrick assembly(SLIC)

Using the SLIC method, infuse the biobricks inside of plasmid. There are two inserts just made through SLIC method.

STEP3.measuring the concentration of heavy metal

This picture is showing cadimum detection. Another case is similar to this experiments.

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+{|style=  "background:#FFCC33;" align="center"
+|
+== Overview ==
+::'''Integrated Heavy Metal Detection System'''
+:::Our team project is designing synthetic modules for simultaneous detection of multiple heavy metals such as
+:arsenic, zinc, and cadmium in E. coli. The ultimate goal is to build a micromachine sensing and determining of
+:the concentration of heavy metals in a sample solution (e.g. the waste water). In order to design the system,
+:we will employ two fluorescence proteins (GFP and RFP) and aryl acylamidase as signal reporters. Since each
+:heavy metal promoter produces unique fluorescence or color by those reporters, if more than two heavy metals
+:coexist in a solution, the results would be interpreted from the convoluted fluorescence and/or color rather
+:than a single signal detection. The successful construction of the synthetic modules in E. coli can be utilized
+:in the form of a lyophilized powder, which can be stored in a drug capsule to make it portable.
+::'''STEP1, Prepare plasmids.'''
+[[Image:KU_plasmids.png|center]]
+::
+::
+::
+::
+::
+::'''STEP2. Gene cloning & Infusion Biobrick assembly(SLIC)'''
+:Using the SLIC method, infuse the biobricks inside of plasmid. There are two inserts just made through SLIC method.
+::::[[Image:insert_yodA_amd.png|center]]
+::::
+[[Image:Insert_ars_znt.png‎|center]]
+::
+::'''STEP3.measuring the concentration of heavy metal'''
+::[[Image:cd2+_detector.png|center]]
+:This picture is showing cadimum detection. Another case is similar to this experiments.
-{|style=  "background:#FFCC33;" align="justify"
-== '''Overview''' ==
-|Project 1 : The bacteria metal sensor to detect three major hamful metals such as arsenic, mercury, lead and cadmium utilizing the law of light mixing among three colors "green, red, blue".
-[[Image:light_mix.png|500px]]
-If we can, additional functioning key can be added.
-The better one has the holding key to hold the data. Holding key can be possible to save the data which contain the concentration of the metal ion in the water.
-Project 2 : Which metal is the most abundant in the water?
-Using tri-stable toggle switch, you can determine which metal is the most abundant.
-Let's say a specific metal is present the most, then assigned light will come out among three colors.
-Project 3: Bacterial navigator
-You are lost and don't have any compass.
-Bacterial navigator can show you which way is north or south.
-|[[Image:detector_model.png|200px|right]]
-== Project Details==
-=== Part 2 ===
-Mercury sensor - Mercury inducing promoter : Part:BBa_I728456 + amd gene
 |}