"
Team:Kyoto/CiC/Notebook/0928-1002
From 2009.igem.org
(Difference between revisions)
(→Monday, 28 September) |
(→Tuesday, 29 September) |
||
| Line 42: | Line 42: | ||
===Tuesday, 29 September=== | ===Tuesday, 29 September=== | ||
====To Do==== | ====To Do==== | ||
| + | *sig-GFP generator | ||
| + | **PCR(the second PCR) | ||
| + | *sig-EGFP generator(HeLa) | ||
| + | **phosphorylation signal sequence primer | ||
| + | **annealing signal sequence primer | ||
| + | **Restriction Enzyme Digestion(EcoR1 + Xho1) | ||
| + | **ligation | ||
====Results==== | ====Results==== | ||
{{:Team:Kyoto/Pad_between_dates}} | {{:Team:Kyoto/Pad_between_dates}} | ||
| + | |||
===Wednesday, 30 September=== | ===Wednesday, 30 September=== | ||
====To Do==== | ====To Do==== | ||
Revision as of 14:21, 21 October 2009
- 0907-0911
- constructing HIV-TAT-(LALAAAA)3-generator
- 0914-0918
- constructing HIV-TAT-(LALAAAA)3-generator
- 0921-0925
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 0928-1002
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1005-1009
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1012-1016
- Observation
- 1019-1023
- Observation
0928-1004 : what to do. what to do. what to do. what to do.
Monday, 28 September
To Do
- sig-EGFP generator(HeLa)
- Restriction Enzyme Digestion(Hind3)
Results
They were unexpectedly digested by Hind3,the RE which would not work if the signal sequence were inserted in the vector correctly.
Tuesday, 29 September
To Do
- sig-GFP generator
- PCR(the second PCR)
- sig-EGFP generator(HeLa)
- phosphorylation signal sequence primer
- annealing signal sequence primer
- Restriction Enzyme Digestion(EcoR1 + Xho1)
- ligation
Results
Wednesday, 30 September
To Do
Results
Thursday, 01 October
To Do
Results
Friday, 02 October
To Do
Results
Saturday, 03 October
To Do
Results
Saturday, 04 October
To Do
Results
Sunday, 05 October
To Do
Results
