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IBB Pune/30 June 2009
From 2009.igem.org
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| + | |||
| + | I) | ||
| + | Got Transformants. Inoculated them in Broth. Isolation Failed. x-( | ||
| + | |||
| + | |||
| + | II) | ||
| + | Took a-MF | ||
| + | >put a non specific PCR with Vf2 and Vr2 | ||
| + | |||
| + | >Gel | ||
| + | |||
| + | >Got Band around 1.3 | ||
| + | |||
| + | >Partial amp occurs. | ||
| + | |||
| + | >proceed with P2f P2r | ||
| + | |||
| + | >Same size amplified. | ||
| + | |||
| + | |||
| + | Conclusion- | ||
| + | |||
| + | PCR does not work to amplify BioBricks. | ||
| + | |||
| + | We have to amplify using TSS Protocol | ||
Latest revision as of 15:16, 21 October 2009
