Team:Kyoto/CiC/Notebook/0921-0925

From 2009.igem.org

(Difference between revisions)
(Monday, 21 September)
(Monday, 21 September)
Line 34: Line 34:
====To Do====
====To Do====
*HIV-TAT-(LALAAAA)3-generator
*HIV-TAT-(LALAAAA)3-generator
-
**miniprep
+
**Colony PCR
-
*TIM23complex signal::EGFP generator
+
-
**miniprep PEGFP-N3 VECTOR (EGFP generator)
+
-
**phosphorylation signal sequence primer
+
====Results====
====Results====
-
*消えた
+
*Binds of expected size were observed.
{{:Team:Kyoto/Pad_between_dates}}
{{:Team:Kyoto/Pad_between_dates}}

Revision as of 16:45, 21 October 2009

  1. Home
  2. CiC
  3. Notebook

0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

0914-0918 : what to do. what to do. what to do. what to do.


Monday, 21 September

To Do

  • HIV-TAT-(LALAAAA)3-generator
    • Colony PCR

Results

  • Binds of expected size were observed.



Tuesday, 22 September

To Do

  • HIV-TAT-(LALAAAA)3-generator
    • miniprep
  • TIM23complex signal::EGFP generator
    • PEGFP-N3 VECTOR (EGFP generator)

Results

  • 消えた



Wednesday, 23 September

To Do

    • ligation
    • transformation

Results

ligation
conc.
NO.Sample nameconc./(ng/ul)
111(stu1)103.2
Transformation

No colony was observed (090917 10:00) 



Thursday, 17 September

To Do

  • Make repetitive sequence (MPR)
  • Transformation(retry)