IBB Pune/1 September 2009

From 2009.igem.org

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(New page: Finally standardized the PCR for a part of the competence gene regulon. Com EF from ''Acinetobacter baylyi'' was successfully amplified and the post PCR cleanup was done. A 16 hr TA ligat...)
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Finally standardized the PCR for a part of the competence gene regulon. Com EF from ''Acinetobacter baylyi'' was successfully amplified and the post PCR cleanup was done.
Finally standardized the PCR for a part of the competence gene regulon. Com EF from ''Acinetobacter baylyi'' was successfully amplified and the post PCR cleanup was done.
A 16 hr TA ligation reaction set up today to ligate the amplicon into standard pGEMT and pCR2.1 vectors.
A 16 hr TA ligation reaction set up today to ligate the amplicon into standard pGEMT and pCR2.1 vectors.

Revision as of 21:09, 21 October 2009




Finally standardized the PCR for a part of the competence gene regulon. Com EF from Acinetobacter baylyi was successfully amplified and the post PCR cleanup was done.

A 16 hr TA ligation reaction set up today to ligate the amplicon into standard pGEMT and pCR2.1 vectors.