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Team:UNIPV-Pavia/Notebook/Week4Sep
From 2009.igem.org
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= Week from September 21st, to September 27th, 2009 = | = Week from September 21st, to September 27th, 2009 = | ||
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<tr> | <tr> | ||
<td align="left" width="50%"> | <td align="left" width="50%"> | ||
| - | <a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week3Sep"> | + | <a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week3Sep#week_start"> |
<img src="https://2009.igem.org/Special:FilePath?file=Left_arrow.png"/> | <img src="https://2009.igem.org/Special:FilePath?file=Left_arrow.png"/> | ||
</a> | </a> | ||
| - | <a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week3Sep">Previous Week</a> | + | <a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week3Sep#week_start">Previous Week</a> |
</td> | </td> | ||
<td align="right" width="50%"> | <td align="right" width="50%"> | ||
| - | <a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week5Sep">Next Week</a> | + | <a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week5Sep#week_start">Next Week</a> |
| - | <a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week5Sep"> | + | <a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week5Sep#week_start"> |
<img src="https://2009.igem.org/Special:FilePath?file=Right_arrow.png"/> | <img src="https://2009.igem.org/Special:FilePath?file=Right_arrow.png"/> | ||
</a> | </a> | ||
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== <html><font class="dayw_style">September, 21st</font></html> == | == <html><font class="dayw_style">September, 21st</font></html> == | ||
| + | *Wiki updating | ||
<div align="right"> | <div align="right"> | ||
| Line 35: | Line 37: | ||
**RBS33 | **RBS33 | ||
**A2 | **A2 | ||
| - | **pNhaA | + | **pNhaA |
| - | * Overnight incubation of | + | * Overnight incubation of these three cultures at 37°C, 220 rpm. |
| + | |||
| + | |||
| + | ''Experiment with Tecan F200'' | ||
| + | *<html><a href="http://aimed11.unipv.it/iGEM2009/TestTECANInfiniteF200/T9002 TEST 22-09-09.pdf" target="_blank">Download Protocol</a></html> | ||
<div align="right"> | <div align="right"> | ||
| Line 44: | Line 50: | ||
== <html><font class="dayw_style">September, 23rd</font></html> == | == <html><font class="dayw_style">September, 23rd</font></html> == | ||
===== pH sensor ===== | ===== pH sensor ===== | ||
| - | 2nd experiment | + | ''2nd experiment'' |
| - | *We put 50 ul into 5 ml LB NaCl 70 mM + Amp pH 6,6 falcon for each of the overnight cultures RBS33 - A2 - pNhaA and incubated them for four hours and a half. | + | *We put 50 ul into 5 ml LB NaCl 70 mM + Amp pH 6,6 falcon for each of the overnight cultures RBS33 - A2 - pNhaA and incubated them for four hours and a half at 37°C, 220 rpm. |
| - | *OD measure of the three | + | *OD measure of the three falcons with TECAN in order to make dilutions with a final volume of 3 ml and an OD equal to 0,02. |
*We diluted: | *We diluted: | ||
**RBS into: | **RBS into: | ||
| Line 63: | Line 69: | ||
*** LB NaCl 70 mM + Amp pH 7,5 | *** LB NaCl 70 mM + Amp pH 7,5 | ||
*** LB NaCl 70 mM + Amp pH 8,5 | *** LB NaCl 70 mM + Amp pH 8,5 | ||
| - | *We started this 6 hours experiment that performed absorbance and fluorescence measures (on three different wells) for each sample every 5 minutes. | + | *We started this 6 hours' experiment that performed absorbance and fluorescence measures (on three different wells) for each sample every 5 minutes. |
| Line 71: | Line 77: | ||
== <html><font class="dayw_style">September, 24th</font></html> == | == <html><font class="dayw_style">September, 24th</font></html> == | ||
| - | + | *Fermentation experiment setup | |
| - | + | *Wiki updating | |
| - | + | ||
<div align="right"> | <div align="right"> | ||
| Line 90: | Line 95: | ||
== <html><font class="dayw_style">September, 26th</font></html> == | == <html><font class="dayw_style">September, 26th</font></html> == | ||
| - | + | *Fermentation experiment | |
| + | *Cloning | ||
| + | *Wiki updating | ||
<div align="right"> | <div align="right"> | ||
[[#top|Top]] | [[#top|Top]] | ||
</div> | </div> | ||
| + | |||
== <html><font class="dayw_style">September, 27th</font></html> == | == <html><font class="dayw_style">September, 27th</font></html> == | ||
===== pH sensor ===== | ===== pH sensor ===== | ||
| Line 101: | Line 109: | ||
**A2 | **A2 | ||
**pNhaA | **pNhaA | ||
| - | *Overnight incubation of | + | *Overnight incubation of these three cultures at 37°C, 220 rpm. |
<div align="right"> | <div align="right"> | ||
| Line 113: | Line 121: | ||
<tr> | <tr> | ||
<td align="left" width="50%"> | <td align="left" width="50%"> | ||
| - | <a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week3Sep"> | + | <a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week3Sep#week_start"> |
<img src="https://2009.igem.org/Special:FilePath?file=Left_arrow.png"/> | <img src="https://2009.igem.org/Special:FilePath?file=Left_arrow.png"/> | ||
</a> | </a> | ||
| - | <a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week3Sep">Previous Week</a> | + | <a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week3Sep#week_start">Previous Week</a> |
</td> | </td> | ||
<td align="right" width="50%"> | <td align="right" width="50%"> | ||
| - | <a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week5Sep">Next Week</a> | + | <a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week5Sep#week_start">Next Week</a> |
| - | <a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week5Sep"> | + | <a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week5Sep#week_start"> |
<img src="https://2009.igem.org/Special:FilePath?file=Right_arrow.png"/> | <img src="https://2009.igem.org/Special:FilePath?file=Right_arrow.png"/> | ||
</a> | </a> | ||
Latest revision as of 23:39, 21 October 2009
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Week from September 21st, to September 27th, 2009
Previous Week
|
Next Week
|
September, 21st
- Wiki updating
September, 22nd
pH sensor
- LB NaCl 70 mM pH 5,5 - 6,6 - 7,5 - 8,5 + Amp preparation.
- Infection of 4 ml LB + Amp with 8 ul from glycerol stock of:
- RBS33
- A2
- pNhaA
- Overnight incubation of these three cultures at 37°C, 220 rpm.
Experiment with Tecan F200
September, 23rd
pH sensor
2nd experiment
- We put 50 ul into 5 ml LB NaCl 70 mM + Amp pH 6,6 falcon for each of the overnight cultures RBS33 - A2 - pNhaA and incubated them for four hours and a half at 37°C, 220 rpm.
- OD measure of the three falcons with TECAN in order to make dilutions with a final volume of 3 ml and an OD equal to 0,02.
- We diluted:
- RBS into:
- LB NaCl 70 mM + Amp pH 5,5
- LB NaCl 70 mM + Amp pH 6,6
- LB NaCl 70 mM + Amp pH 7,5
- LB NaCl 70 mM + Amp pH 8,5
- pNhaA into:
- LB NaCl 70 mM + Amp pH 5,5
- LB NaCl 70 mM + Amp pH 6,6
- LB NaCl 70 mM + Amp pH 7,5
- LB NaCl 70 mM + Amp pH 8,5
- A2 into:
- LB NaCl 70 mM + Amp pH 5,5
- LB NaCl 70 mM + Amp pH 6,6
- LB NaCl 70 mM + Amp pH 7,5
- LB NaCl 70 mM + Amp pH 8,5
- RBS into:
- We started this 6 hours' experiment that performed absorbance and fluorescence measures (on three different wells) for each sample every 5 minutes.
September, 24th
- Fermentation experiment setup
- Wiki updating
September, 25th
pH sensor
- Preparation of LB NaCl 151 mM at pH 5,5 - 6,6 - 7,5 - 8,5.
- Preparation of LB NaCl 250 mM at pH 5,5 - 6,6 - 7,5 - 8,5.
September, 26th
- Fermentation experiment
- Cloning
- Wiki updating
September, 27th
pH sensor
- Infection of 4 ml LB + Amp with 8 ul from glycerol stock of:
- RBS33
- A2
- pNhaA
- Overnight incubation of these three cultures at 37°C, 220 rpm.
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Previous Week
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Next Week
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