"
Team:UNICAMP-Brazil/Notebooks/September 11
From 2009.igem.org
(Difference between revisions)
(→ColiGuard) |
(→PY Promoter - PY1 and PY2 purification) |
||
| (2 intermediate revisions not shown) | |||
| Line 10: | Line 10: | ||
*<p style=”text-align:justify;”>We purified the PCR products PY1 and PY2 with the Invitrogen's PureLink™ PCR Purification Kit, following the manufacturer's protocol without modifications. | *<p style=”text-align:justify;”>We purified the PCR products PY1 and PY2 with the Invitrogen's PureLink™ PCR Purification Kit, following the manufacturer's protocol without modifications. | ||
| - | '' | + | ''Fabi and Léo'' |
==''' YeastGuard '''== | ==''' YeastGuard '''== | ||
====New biobricks in biobrick format==== | ====New biobricks in biobrick format==== | ||
| - | + | ||
| + | The four ligation reactions that were reacting O/N were dialyzed for one hour before undergoing transformation into ''E. coli'' ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Electroporationi Protocol 3]). 5µl of each ligation were used to transform the bacterias. After growing at 37ºC for one hour in LB medium, they were plated on LB+AMP plates and continue to grow at 37ºC O/N.</p> | ||
''Taís'' | ''Taís'' | ||
{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} | ||
Latest revision as of 23:56, 21 October 2009
| ||||||||||||||||||||||||||||||||||
