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Team:UNICAMP-Brazil/Notebooks/September 11
From 2009.igem.org
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__NOTOC__ | __NOTOC__ | ||
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| + | ==''' ColiGuard '''== | ||
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| + | ==== PY Promoter - PY1 and PY2 purification ==== | ||
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| + | *<p style=”text-align:justify;”>We purified the PCR products PY1 and PY2 with the Invitrogen's PureLink™ PCR Purification Kit, following the manufacturer's protocol without modifications. | ||
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| + | ''Fabi and Léo'' | ||
==''' YeastGuard '''== | ==''' YeastGuard '''== | ||
====New biobricks in biobrick format==== | ====New biobricks in biobrick format==== | ||
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| + | The four ligation reactions that were reacting O/N were dialyzed for one hour before undergoing transformation into ''E. coli'' ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Electroporationi Protocol 3]). 5µl of each ligation were used to transform the bacterias. After growing at 37ºC for one hour in LB medium, they were plated on LB+AMP plates and continue to grow at 37ºC O/N.</p> | ||
''Taís'' | ''Taís'' | ||
{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} | ||
Latest revision as of 23:56, 21 October 2009
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