Team:UNICAMP-Brazil/Notebooks/August 12

From 2009.igem.org

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{{:Team:UNICAMP-Brazil/inc calendar}}
{{:Team:UNICAMP-Brazil/inc calendar}}
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==Coliguard: mini-prep==
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__NOTOC__
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Today we performed mini-preps from yesterday´s cultures according to protocol 2 (see prototol section). We recovered all plasmids!!!
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==''' ColiGuard '''==
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==YeastGuard: Mini-prep==
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====Miniprep====
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Only 4 of the 6 cultures grew in the liquid medium. We performed the plasmid extraction of these 4 cultures.
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*<p style=”text-align:justify;”>Today we performed minipreps from yesterday´s cultures according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2]. We recovered all plasmids!!!</p>
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The protocol used was "Protocols - 2".
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==''' YeastGuard '''==
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'''Taís'''
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====Recovering the biobricks====
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*<p style=”text-align:justify;”>We performed the plasmid extraction of the 4 cultures that grew in the liquid medium ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2]).</p>
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*<p style=”text-align:justify;”>One of the colonies that didn´t work was from the same biobrick we had problems before: BBa_J63002.</p>
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''Taís''
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Latest revision as of 00:40, 22 October 2009

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ColiGuard

Miniprep

  • Today we performed minipreps from yesterday´s cultures according to Protocol 2. We recovered all plasmids!!!

YeastGuard

Recovering the biobricks

  • We performed the plasmid extraction of the 4 cultures that grew in the liquid medium (Protocol 2).

  • One of the colonies that didn´t work was from the same biobrick we had problems before: BBa_J63002.

Taís