Team:UNICAMP-Brazil/Notebooks/August 13

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(New page: ==Coliguard: recovering the BioBricks== Today we ressuspended the chosen BioBricks according to the standard protocol. Each undergraduated student was responsible to ressuspend a set of B...)
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==Coliguard: recovering the BioBricks==
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{{:Team:UNICAMP-Brazil/inc_topo}}
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{{:Team:UNICAMP-Brazil/inc calendar}}
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__NOTOC__
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Today we ressuspended the chosen BioBricks according to the standard protocol. Each undergraduated student was responsible to ressuspend a set of BioBricks. All of them were transfomed into electrocompetent E. coli (DH10B) according to protocol 3 (see protocol section). The cells were then plated in LB media containing the appropriate antibiotic.
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==''' ColiGuard '''==
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'''Marcelo'''
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====Recovering the BioBricks====
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Part:BBa_B0030 - RBS.1 (strong) -- modified from R. Weiss 
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*<p style=”text-align:justify;”>Today we resuspended 4 BioBricks:</p>
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Part:BBa_B0032 - RBS.3 (medium) -- derivative of BBa_0030
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BBa_K131009
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'''Luige'''
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BBa_K117002
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Part:BBa_J61100 -  Ribosome Binding Site Family Member 
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BBa_J04430
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Part:BBa_J61107 - Ribosome Binding Site Family Member
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BBa_K091109
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'''Marcos'''
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*<p style=”text-align:justify;”>We transformed the biobricks into ''E. coli'' DH10B competent cells and plated on adecuated antibiotic plates.</p>
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Part:BBa_B0010 - T1 from E. coli rrnB
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''Luige''
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Part:BBa_B0014 - double terminator
 
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'''Leonardo'''
 
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Part:BBa_B0015 - double terminator (B0010-B0012) 
 
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Part:BBa_R0040 -  TetR repressible promoter
 
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Part:BBa_J23100 - constitutive promoter family member
 
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Part:BBa_J23107 - constitutive promoter family member
 
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'''Fabiana'''
 
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Part:BBa_J23109 - constitutive promoter family member
 
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Part:BBa_J31007 - tetracycline resistance protein TetA(C)
 
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Part:BBa_J31003 - kanamycin resistance forward
 
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Part:BBa_E0040 - green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212)
 
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'''Victor'''
 
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Part:BBa_E1010: **highly** engineered mutant of red fluorescent protein from Discosoma striata
 
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Part:pSB1A3 -  High copy BioBrick assembly plasmid
 
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Part:pSB1AK3 - High copy BioBrick assembly plasmid
 
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Part:pSB3K3 -  Low to medium copy BioBrick standard vector
 
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Part:pSB6A1 - pBR322 converted to BioBrick vector (formally BBa_I739201)
 
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Latest revision as of 00:45, 22 October 2009

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ColiGuard

Recovering the BioBricks

  • Today we resuspended 4 BioBricks:

BBa_K131009

BBa_K117002

BBa_J04430

BBa_K091109

  • We transformed the biobricks into E. coli DH10B competent cells and plated on adecuated antibiotic plates.

Luige