Team:UNICAMP-Brazil/Notebooks/August 21

(Difference between revisions)

Latest revision as of 01:00, 22 October 2009

Still fighting for RBS

We digested more plasmids with the SpeI and PstI enzymes (Protocol 14). We left it digesting a little longer and put less amount of DNA into the reaction. This time it seamed to work.

Raíssa

@@ Line 6: / Line 6: @@
 ==''' YeastGuard '''==
-====Recovered biobricks====
-'''Miniprep, digestion and ligation of RBS'''
+'''Still fighting for RBS'''
-* Today we perfomed miniprep from the RBS transformed ''E. coli'' according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2]. The fragment size seamed to be correct and we decided to digest it according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Digestion_reaction Protocol 14]  with the ''Spe''I and ''Pst''I enzymes. It worked.
+* We digested more plasmids with the ''Spe''I and ''Pst''I enzymes ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Digestion_reaction Protocol 14]). We left it digesting a little longer and put less amount of DNA into the reaction. This time it seamed to work.
-* After the RBS plasmid digestion, we ligated it to CFP previously digested with ''Xba''I and ''Pst''I according to the  [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/T4_DNA_Ligase Protocol 11].
+[[image:20090822_Rá_digestao 2 rbs 22_08_09.JPG|150px|center]]
-[[Image:RBSdigestion.jpg‎|500px|center]]
 ''Raíssa''
 {{:Team:UNICAMP-Brazil/inc_rodape}}