Team:UNICAMP-Brazil/Notebooks/August 21

From 2009.igem.org

(Difference between revisions)
(→YeastGuard)
 
(16 intermediate revisions not shown)
Line 1: Line 1:
-
== YeastGuard: Mini-prep, digestion and ligation of RBS ==
+
{{:Team:UNICAMP-Brazil/inc_topo}}
 +
{{:Team:UNICAMP-Brazil/inc calendar}}
-
- Today we perfomed mini-prep from the RBS transformed ''E. coli''. The fragment syze was correct,we decided to digest it without purifying and it worked.
+
__NOTOC__
-
- Afetr the RBS plasmid digestion we ligated it to CFP (the reporter gene) according to the protocol W.
+
==''' YeastGuard '''==
-
'''Raíssa'''
+
'''Still fighting for RBS'''
 +
 
 +
* We digested more plasmids with the ''Spe''I and ''Pst''I enzymes ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Digestion_reaction Protocol 14]). We left it digesting a little longer and put less amount of DNA into the reaction. This time it seamed to work.
 +
 
 +
[[image:20090822_Rá_digestao 2 rbs 22_08_09.JPG|150px|center]] 
 +
 
 +
''Raíssa''
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Latest revision as of 01:00, 22 October 2009

Topo l2.gif topo_r_igem.gif
topo_r_b.gif
Back to Calendar

YeastGuard

Still fighting for RBS

  • We digested more plasmids with the SpeI and PstI enzymes (Protocol 14). We left it digesting a little longer and put less amount of DNA into the reaction. This time it seamed to work.
20090822 Rá digestao 2 rbs 22 08 09.JPG

Raíssa




Retrieved from "http://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/August_21"