Team:UNICAMP-Brazil/Notebooks/August 21

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==''' YeastGuard '''==
==''' YeastGuard '''==
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====Miniprep, digestion and ligation of RBS====
 
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* Today we perfomed miniprep from the RBS transformed ''E. coli''. The fragment size was correct and we decided to digest it without purifying. It worked.
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'''Still fighting for RBS'''
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* After the RBS plasmid digestion, we ligated it to CFP (the reporter gene) according to the Protocol W.
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* We digested more plasmids with the ''Spe''I and ''Pst''I enzymes ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Digestion_reaction Protocol 14]). We left it digesting a little longer and put less amount of DNA into the reaction. This time it seamed to work.
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[[image:20090822_Rá_digestao 2 rbs 22_08_09.JPG|150px|center]] 
''Raíssa''
''Raíssa''
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Latest revision as of 01:00, 22 October 2009

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YeastGuard

Still fighting for RBS

  • We digested more plasmids with the SpeI and PstI enzymes (Protocol 14). We left it digesting a little longer and put less amount of DNA into the reaction. This time it seamed to work.
20090822 Rá digestao 2 rbs 22 08 09.JPG

Raíssa




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